RBS
Part:BBa_K4192000:Design
Designed by: Muhua Liu Group: iGEM22_CAU_China (2022-09-27)
Strong RBS of Pseudomonas fluorescens
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Unlike most RBS, it is only five bases long, so attention should be paid to the spacing in practice to avoid possible frameshift mutations.
Source
It is obtained by improving the RBS sequence (BBa_K4192002) in the original P.fluorescen.